Composite

Part:BBa_K180006

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-16)

Game of Life - Primary plasmid (part 2) [lux pR, GFP and LacI generator]

Part of the iGEM2009 Team:Southampton Game of Life primary plasmid.

LacI and GFP-LVA are generated under lux pR regulation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1228
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1904


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/bidirectional
//function/reporter/fluorescence
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
controllux
directionforward
emission511 nm
emit511
excitation501 nm
excite501
lum
n/aGame of Life - Primary plasmid (part 2) [lux pR, GFP and LacI generator]
proteinGFPmut3b
tagLVA